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Anti-GAPDH Mouse Monoclonal Antibody
Tagged proteins can be detected and/or purified using highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to each individual protein. In this regard, Tagging Antibodies can be used as universal antibodies. There are several epitope tags commonly used, such as 6-histidine (6X-His), c-myc (Myc), influenza A virus haemagglutinin (HA) and glutathione-S-transferase (GST). Normalizing Antibodies are very useful to check if the same amount of protein samples are loaded onto each lane. This is especially true for quantitative comparisons by Western experiment.
SPECIAL FEATURES
- Mouse Monoclonal (IgG1)
- Recognizes many species
APPLICATIONS
- Loading control for Western Blot 1:1000
- Immunofluorescence experiments 1:100
Application of the GAPDH antibody as a protein loading control in Western blots. Total proteins for HeLa, Jurkat and Daudi were blotted with GAPDH monoclonal antibody. Immunoflorescence staining of GAPDH in SH-SY5Y cells, counterstained with Hoechst DNA dye.
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