Anti-His Tag Mouse Monoclonal Antibody
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by tagged genes. Tagged proteins can be detected and/or purified using highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to each individual protein. In this regard, Tagging Antibodies can be used as universal antibodies. There are several epitope tags commonly used, such as 6-histidine (6X-His), c-myc (Myc), influenza A virus haemagglutinin (HA) and glutathione-S-transferase (GST). Normalizing Antibodies are very useful to check if same amount of protein samples is load on each lane. This is especially true for quantitative comparisons by Western experiment. This mouse monoclonal IgG2b is a 6x His synthetic peptide.
SPECIAL FEATURES
- Recognizes His-tagged recombinant proteins or His-tagged over-expressed cells.
APPLICATIONS
- ELISA, Dot Blot, Western Blotting (1:1000), recommended start.
- Immunoprecipitation and Immunostaining (1:200), recommended start.
His Western blot analysis of 293 cells transfected with His-tagged vector (2) and untransfected control (1).
Immunoflurescence staining of Hi-tag fusion protein in 293 cells.
A. Untransfected controlB. Transfected
| Item # | NAME | SIZE | UNIT PRICE | Qty |  Cart |
| G020 | Anti-His Tag Mouse Monoclonal Antibody | 100 ug | $85.00 | - BUY |
| PROTOCOL |