Anti-MBP Tag Rabbit Polyclonal Antibody
Anti-MBP is optimally suited for monitoring the expression of MBP tagged fusion proteins. As such, anti- MBP/MBP can be used to identify fusion proteins containing the MBP epitope. The antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against MBP containing recombinant proteins.
Although not tested for immunoprecipitation and immunocytochemistry, and other immunodetection techniques, this antibody is likely functional. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for easy purification from bacterial extracts under mild conditions. Anti-MBP is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli. By Western blot, a band is seen at ~42 KDa; representing MBP.
SPECIAL FEATURES
- Recognizes the 42KDa band in Western Blotting
- Rabbit Polyclonal
- Used for Human and Mouse species
APPLICATIONS
- Western Blot Analysis 1:1000
- Immunoprecipitation 5ug/mg lysate
- Histo/Cytochemistry 1:200
Anti-MBP epitope tag polyclonal antibody detects MBP-tagged recombinant proteins by Western blot.
Polyclonal rabbit-Anti-MBP epitope tag at 0.5-1.0 ug/ml was used to detect 1.0ug of recombinant protein containing the MBP epitope tag. a 4-20%gradient get was used to separate the protein by SDS-Page.
| Item # | NAME | SIZE | UNIT PRICE | Qty |  Cart |
| G079 | Anti-MBP Tag Rabbit Polyclonal Antibody | 100 ug | $195.00 | - BUY |
| PROTOCOL |