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DNA Walking SpeedUp™ PreMix Kit
Several PCR techniques have been developed to obtain unknown sequences located adjacent to a known sequence without having to construct a DNA library or screen clones. These PCR methods include PCR (IPCR), ligation-mediated PCR (LM-PCR) and randomly primed PCR (RP-PCR). Although these methods have proven to be effective, they are still hampered by laborious steps such as restriction enzyme digestion, adapter ligation and purification procedures. Moreover, arbitrary primers in RP-PCR often result in spurious products because of non-specific annealing. These drawbacks to existing genomic walking methods can be overcome with Annealing Control Primer (ACP™) technology, which enhances the specificity of short oligomers. ACP™ technology makes DNA walking experiments much simpler and more cost-effective.
SPECIAL FEATURES
- No complex processes, such as restriction digestion or immobilization
- Generate products in less than 1 day
- 10 times faster than other methods
- No library construction, no library screening and no sub-cloning
- No false products
APPLICATIONS
- Identification of promoter regions
- Transgene localization
- Detection of deletions, insertions, or isoforms
- Isolation of unknown sequences adjacent to known sequences
