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EasyScript™ Reverse Transcriptase
EasyScript™ Reverse Transcriptase is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV) with minimal RNase H activity. The enzyme is purified from bacteria host as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.
APPLICATIONS
- Synthesis cDNA from a single-stranded RNA or DNA Primer extension
- Sequencing dsDNA
- cDNA library
- Template production for use in PCR
- 3'-end labeling of duplex DNA via end-filling reactions
