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Ni-Agarose-N5 Resin
Ni-Agarose-N5 Resin is widely used for purification of proteins with an affinity tag of six consecutive histidine residues—the 6x His-tag. Because of its remarkable binding selectivity for 6xHis tag, Ni-Agarose-N5 Resin makes one-step purification possible for almost any His-tagged protein, from any expression system, under native or denaturing conditions. The Ni-Agarose-N5 Resin is supplied precharged with Ni2+ ions. Proteins with 6xHis tag bind to the resin. Impurities such as unwanted proteins will wash away and purified His-tagged proteins can be eluted by imidazole or reduced pH solution.
Imidazole at low concentrations can be included in binding and wash buffers to minimize the non-specific binding of unwanted host cell proteins; however, imidazole may also decrease the binding of histidine-tagged proteins; therefore, the imidazole concentration must be optimized to ensure the best balance of high purity and high yield. Optimal concentration of imidazole is protein-dependent and therefore must be determined by experiment.
SPECIAL FEATURES
- Remarkable binding selectivity
- One-step purification
- Precharged with Ni2+ ions
