Products > Protein Research Reagents >Ni-Agarose-N5 Resin

Protein Research Reagents Ni-Agarose-N5 Resin

Ni-Agarose-N5 Resin is widely used for purification of proteins with an affinity tag of six consecutive histidine residues—the 6xHis tag. Because of its remarkable binding selectivity for 6xHis tag, Ni-Agarose-N5 Resin makes one-step purification possible for almost any His-tagged protein, from any expression system, under native or denaturing conditions. The Ni-Agarose-N5 Resin is supplied precharged with Ni2+ ions. Proteins with 6xHis tag bind to the resin. Impurities such as unwanted proteins will wash away and purified His-tagged proteins can be eluted by imidazole or reduced pH solution.

Imidazole at low concentrations can be included in binding and wash buffers to minimize the non-specific binding of unwanted host cell proteins; however, imidazole may also decrease the binding of histidine-tagged proteins; therefore, the imidazole concentration must be optimized to ensure the best balance of high purity and high yield. Optimal concentration of imidazole is protein-dependent and therefore must be determined by experiment.

Special offer - Buy any size Ni-Agarose-N5 Resin and receive 2 FREE Gravity-Flow Columns, Cat. No. G20C with your order! Offer ends 8/31/10.

SPECIAL FEATURES
  • Remarkable binding selectivity
  • One-step purification
  • Precharged with Ni2+ ions

 
Item # NAME SIZE UNIT PRICE   Qty Cart
G201 Ni-Agarose-N5 Resin 5 mL $55.00      - BUY
G202 Ni-Agarose-N5 Resin 25 mL $195.00      - BUY
G203 Ni-Agarose-N5 Resin 100 mL $625.00      - BUY
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