One-Shot™ PCR Master Mixes
These are the individual One-Shot™ PCR Master Mixes from the One-Shot™ PCR Kit (D125). The One-Shot™ PCR Kit contains 12 different PCR Master Mixes formulated with multiple thermal DNA polymerases and pre-optimized PCR buffers and enhancers in 12 different combinations. All the Master Mixes are in the 2X format. The One-Shot™ PCR Kit simplifies the process to determine optimal conditions for each unique combination of template and primers, and works well for regular PCR and special PCRs; such as long PCR, high GC PCR and PCR with low abundant templates. Once the optimal conditions are determined, individual Master Mixes can be used for future experiments.
The 2XMaster Mix format means that users only need to add their template, primers, and H2O, if needed. All the Master Mixes contain an inert red dye which allows reaction products to be loaded directly into a gel for electrophoresis without the need for loading buffer.
SPECIAL FEATURES
- Multiple DNA polymerases and enhancers
- All-in-one 2X Master Mix format; ready to use
- New generation PCR concept and technologies
- Get results every time
APPLICATIONS
- Easily find optimal PCR conditions
- General PCR, high GC and long PCR
- Specific amplification of complex templates
- Genotyping
- High-throughput screening
Amplification of different DNA fragments using the One-Shot™ PCR Kit.
1. A 2.5kb fragment amplified from a cloned mouse gene.
2. A 2.5kb fragment amplified from mouse genomic DNA.
3. A 0.7kb GAPDH fragment amplified from mouse genomic DNA.
4. A 0.7kb TCRD fragment amplified from mouse genomic DNA.
| Item # | NAME | SIZE | UNIT PRICE | Qty |  Cart |
| D125-01 | One-Shot™ PCR Master Mixes | 200x50ul rxns | $145.00 | - BUY | |
| D125-02 | One-Shot™ PCR Master Mixes | 200x50ul rxns | $145.00 | - BUY | |
| D125-03 | One-Shot™ PCR Master Mixes | 200x50ul rxns | $145.00 | - BUY | |
| D125-04 | One-Shot™ PCR Master Mixes | 200x50ul rxns | $145.00 | - BUY | |
| D125-05 | One-Shot™ PCR Master Mixes | 200x50ul rxns | $145.00 | - BUY | |
| D125-06 | One-Shot™ PCR Master Mixes | 200x50ul rxns | $145.00 | - BUY | |
| D125-07 | One-Shot™ PCR Master Mixes | 200x50ul rxns | $145.00 | - BUY | |
| D125-08 | One-Shot™ PCR Master Mixes | 200x50ul rxns | $145.00 | - BUY | |
| D125-09 | One-Shot™ PCR Master Mixes | 200x50ul rxns | $145.00 | - BUY | |
| D125-10 | One-Shot™ PCR Master Mixes | 200x50ul rxns | $145.00 | - BUY | |
| D125-11 | One-Shot™ PCR Master Mixes | 200x50ul rxns | $145.00 | - BUY | |
| D125-12 | One-Shot™ PCR Master Mixes | 200x50ul rxns | $145.00 | - BUY |
| PROTOCOL | MSDS |
Technical Tip
Specific amplification parameters are dependent on particular primer/template combinations; to start with, users commonly employ conditions similar to the following: denature at 94°C for 1 minute, anneal at 55°C for 1 minute, and perform the primer extension at 72°C for 1 minute/kb, for a total number of 35 cycles. As does the reaction mix itself, relative G/C content will vary depending on the templates and primers. Dealing with an unknown template in the primer sequence, will affect annealing temperature—if an appropriate temperature is not known for a primer pair, a quick estimate of 2ºC for each A/T and 4ºC for each G/C in the primer sequence is a good place to start. Once the optimal conditions and particular Master Mix are identified, use them for future reactions. Call us at 1-800-631-5009 should you have questions or need help.