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PuRetro™ Lentivirus and Retrovirus Purification Kits
Traditionally, both recombinant retrovirus and lentivirus have been concentrated via ultracentrifugation. Although the method works well, it requires specific ultracentrifugation equipment and it is technically demanding. In addition, the total viral supernatant volume is limited to the size of ultracentrifugation tubes. Reports have also stated that the ultracentrifugation process has some detrimental physical effects on the recombinant virus.
Due to limitations on the existing technique, there is a great necessity for a quick and efficient method to purify and concentrate recombinant retrovirus and lentivirus. The PuRetro™ system is based on an ion-exchange filter membrane, and has proved to be the most effective retroviral and lentiviral purification method of its kind.
The PuRetro™ method proves to be fast, efficient and reliable. Moreover, it is capable of conveniently processing large volumes of viral supernatant (up to 150ml using the Giga kit). Depending on the amount of viral supernatant input and the final elution amount of viral stock, a 50x to 150x fold increase in viral concentration can be achieved using this method.
SPECIAL FEATURES
- No detrimental physical effects to virus.
- Fast, efficient and reliable.
- High concentrations can be achieved.
APPLICATIONS
- Retrovirus and lentivirus purification.
- In vitro and in vivo gene delivery.
