Easy-to-use and economical; choose guaranteed products from Lamda Biotech. Choose from a wide variety of products; such as Protein Markers, BCA Protein Assay, Bradford Protein Assay, Ni-Agarose-N5 Resin for 6xHis-tagged Protein Purification, and Western Blot Detection System.
Widely used for purification of proteins with an affinity tag of six consecutive histidine residues—the 6xHis tag. Its remarkable binding selectivity for 6x His-Tag makes one-step purification possible for almost any His-tagged protein.
This assay is based on the equilibrium between three forms of Coomassie Blue G dye. Under acidic conditions, proteins bind to the reagent, causing a spectral shift from reddish/brown to blue.
The principle of the bicinchoninic acid (BCA) assay is similar to the Lowry procedure, in that both rely on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+.