The One-Step TaqProbe qRT-PCR Kit is a complete quantitative RT-PCR kit for TaqMan assay in one step. It contains all reagents necessary for both Reverse Transcription (RT) and TaqMan Probe based qPCR amplification to occur in a single qPCR reaction tube.

The One-Step TaqProbe qRT-PCR Kit is an integration of two key processes: the RT and the real-time PCR. With our innovative RT Master Mix, stabilizers and enhancers, and the hot-start qPCR enzyme, it enables a seamless coupling of two separate steps into one real-time “single step” process. It is very easy and useful for gene expression study and other practical applications. This kit works well on most of the real-time PCR machines.

Kit Contents

• TaqProbe qPCR MasterMix: 1250 µl
  
• qRT-PCR Enzyme Mix: 40 µl
• ROX dye solution:: 15 µl
• Nuclease-free H2O: 1000 µl