PreSafeStain 6X DNA Loading Dye
The principle of the bicinchoninic acid (BCA) assay is similar to the Lowry procedure, in that both rely on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. In the second step, BCA forms a purple colored complex with Cu+1, which is detectable at 562 nm.
Protein + Cu2+ => Cu1+ + BCA => Cu1+- BCA complex
It has been shown that cysteine, cystosine, tryptophan, tyrosine, and the peptide bond are able to reduce Cu2+ to Cu1+. BCA forms a purple-blue complex with Cu1+ in alkaline environments, thus providing a basis to monitor the reduction of alkaline Cu2+ by proteins at absorbance maximum 562 nm.
|Catalog #||Name||Size||Unit Price||Qty|
|G1002||BCA Protein Assay (Kit)||1 kit||$120.00|
|G075||ECL Western Blotting Detection Kit||200ml||$90.00|
|PROT-SC||Shredder Spin Column With Cap||100/set||$90.00|
|PROT-SC-N||Shredder Spin Column||100/set||$90.00|
|WM1000||Western Protein Marker||250ul||$165.00|
|G02103||AccuRuler Broad Prestained Protein Ladder||2 x 250ul||$135.00|
|G10180||AccuRuler Prestained Protein Ladder II||2x250µl||$105.00|