• BCA Protein  Assay (Kit)


The principle of the bicinchoninic acid (BCA) assay is similar to the Lowry procedure, in that both rely on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. In the second step, BCA forms a purple colored complex with Cu+1, which is detectable at 562 nm.


Protein + Cu2+ => Cu1+ + BCA => Cu1+- BCA complex


It has been shown that cysteine, cystosine, tryptophan, tyrosine, and the peptide bond are able to reduce Cu2+ to Cu1+. BCA forms a purple-blue complex with Cu1+ in alkaline environments, thus providing a basis to monitor the reduction of alkaline Cu2+ by proteins at absorbance maximum 562 nm.


SPECIAL FEATURES

  • Simple and sensitive in protein assays.
  • Faster and easier than Lowry protein assay.
  • Compatible with many ionic and nonionic detergents.
  • Independent of specific amino acid content of proteins.
  • Accurate over a wide range of protein concentrations.

KIT CONTENTS

  • BCA Protein Assay Reagent 1: 750ml
  • BCA Protein Assay Reagent 2: 25ml
  • Protein Standard Solution: 10ml


Protocols


BCA Protein Assay (Kit)

  • Catalog#: G1002
  • $120.00


Available Options

Size Unit Price Qty    
1 kit

$120.00


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