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EasyScript™ cDNA Synthesis Kit
EasyScript™ First Strand cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates. The recombinant RNasin™ Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation. The kit is also supplied with both oligo(dT) and random primers. The oligo(dT) anneals selectively on the poly(A) tail of mRNA. Random primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5’-end regions of mRNA. Gene-specific primers may also be used with the kit. The first strand of cDNA can be directly used as a template in PCR. The EasyScript™ Reverse Transcriptase (RTase) within the kit, is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV) with minimal RNase H activity. The enzyme is purified from bacteria host as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.
APPLICATIONS
- First strand cDNA synthesis for PCR
- Construction of cDNA libraries
- Generation of probes for hybridization
