Real-Time PCR (qPCR) Guide

August 14, 2019

Basically, there are three kinds of real-time PCR (qPCR): ONE-STEP REAL-TIME PCR Use purified RNA. The two reactions of cDNA synthesis by RT (reverse transcription) and real-time PCR happen in one tube. Fewer steps reduce possible contamination. Less pipetting ensures …

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For your cDNA-qPCR research, consider this

August 13, 2019

Comparing side-by-side to the leader, we make your selection easy for high-quality cDNA-qPCR reagents:    THERMO FISHER PRODUCT   LAMDA PRODUCT      SuperScript™ II Reverse Transcriptase (Cat# 18064014) List Price: 10,000U, $355   EasyScript™ Reverse Transcriptase (Cat# G231) List Price: 5,000U, $85   SuperScript™ III Reverse Transcriptase (Cat# …

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Are you still using EthBr?

June 18, 2018

Ethidium Bromide (EthBr) is a potent mutagen that is commonly used as fluorescent dye used in DNA research in molecular biology labs. EthBr intercalates into DNA, inserting itself between bases, which affects DNA biological processes like replication and transcription. Lamda …

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What are the benefits of Direct PCR?

June 18, 2018

Direct PCR allows for direct amplification from the tissue source without the need for DNA purification. This allows time to be saved, contamination to decrease, and prevents sample loss. Direct PCR is great for low amount of DNA as well …

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DNA Gel Essentials

July 19, 2017

-Our popular DNA Ladders have a great reputation for their consistent results. That’s why at Lamda Biotech we have an array of DNA Ladders that make it easy to determine size with clear sharp bands. They are all premixed, ready-to-load and come …

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