Real-Time PCR (qPCR) Guide

August 14, 2019

Basically, there are three kinds of real-time PCR (qPCR): ONE-STEP REAL-TIME PCR Use purified RNA. The two reactions of cDNA synthesis by RT (reverse transcription) and real-time PCR happen in one tube. Fewer steps reduce possible contamination. Less pipetting ensures …

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For your cDNA-qPCR research, consider this

August 13, 2019

Comparing side-by-side to the leader, we make your selection easy for high-quality cDNA-qPCR reagents:    THERMO FISHER PRODUCT   LAMDA PRODUCT      SuperScript™ II Reverse Transcriptase (Cat# 18064014) List Price: 10,000U, $355   EasyScript™ Reverse Transcriptase (Cat# G231) List Price: 5,000U, $85   SuperScript™ III Reverse Transcriptase (Cat# …

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Are you still using EthBr?

June 18, 2018

Ethidium Bromide (EthBr) is a potent mutagen that is commonly used as fluorescent dye used in DNA research in molecular biology labs. EthBr intercalates into DNA, inserting itself between bases, which affects DNA biological processes like replication and transcription. Lamda …

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What are the benefits of Direct PCR?

June 18, 2018

Direct PCR allows for direct amplification from the tissue source without the need for DNA purification. This allows time to be saved, contamination to decrease, and prevents sample loss. Direct PCR is great for low amount of DNA as well …

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Direct-Cell™ Gene Expression Kits

June 05, 2017

—Direct RT-qPCR Kits provides a complete line of reagents for real-time RT-PCR analysis directly from cultured cells without RNA purification, which makes gene expression that quick and easy. At Lamda we offer our customers direct RT-qPCR kits that can be …

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RT™ Master Mix

May 30, 2017

5X RT MasterMix Kits include all the reagents needed in a ready-to-use format that saves on preparation time, while also reducing the risk of contamination.  With our competitive pricing time is not the only thing saved! For consistent results that …

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DIRECT™PCR Kits

May 28, 2017

– – In addition to our TISSUE-DIRECT™PCR Kit (Cat. No. D300), we are pleased to announce our, BAC-DIRECT™PCR Kit (Cat. No. D301), PLANT-DIRECT™PCR Kit (Cat. No. D302, SEED-DIRECT™PCR Kit (Cat. No. D303), BEYOND-BLOOD™ DIRECT PCR Kit (D304) BAC-DIRECT™PCR Kit (D301) …

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