• RT™ All-in-One Master Mix, 5X


The 5X RT™ All-in-One Master Mix is a ready-to-use master mix for first-strand cDNA synthesis in a 5X concentration. It contains the EasyScriptTM RTase, ribonuclease inhibitor, cDNA synthesis enhancers, dNTPs, and a balanced concentration for oligo(dT) and random primers. The oligo(dT) anneals selectively to the poly(A) tail of mRNAs and the random primers will anneal anywhere of a RNA molecule. The resultant cDNA can be directly used as template in different experiments, such as PCR.   

EasyScript Reverse Transcriptase, or called EasyScript™ RTase, is a novel engineered reverse transcriptase with higher efficiency in the first-strand cDNA synthesis from RNA templates. It represents the best performing RTase on the market. Due to a series of mutations introduced into the RNase H domain of this enzyme, there is no detectable RNase H activity for this enzyme, resulting in better yield and length of synthesized cDNA. Furthermore, EasyScript™ RTase contains an additional fidelity-enhancing subunit which drastically enhances accuracy in reverse transcription.


SPECIAL FEATURES

  • Up to 9kb cDNA synthesis
  • Ensures sample to sample consistency
  • Large RNA sample volume capacity
  • Low concentration RNA samples

APPLICATIONS

  • cDNA synthesis for PCR
  • Construction of cDNA libraries
  • cDNA cloning

Protocols


RT™ All-in-One Master Mix, 5X

  • Catalog#: G208-100
  • $125.00


Available Options

Size Unit Price Qty    
100rxn

$125.00


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