The Accu Prestained Protein Ladder  contains 10 prestained proteins, covering a wide range of molecular weights from 8.5kDa to 180kDa. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins. The ladder is supplied in protein gel loading buffer and is ready-to-use. No need to heat, dilute, or add reducing agent before loading.

SPECIAL FEATURES

• Sharp and stable

• 2 reference bands - red at ~70kDa and green at ~25kDa

• Ready to use - no need to boil

• Broad range

• For each application use  about 5µl for each well, depending on the size of the wells, for clear visualization during electrophoresis

APPLICATIONS

• Monitoring protein migration during polyacrylamide gel electrophoresis

• Monitoring protein transfer onto membranes during Western blotting

• Sizing of proteins on polyacrylamide gels and Western blots

Usage: For each application, 3 - 5µl is needed, depending on the size of the wells.

Size: 500µl

Storage: Store at -20°C (Room temp stable for short term)

Contents: 0.1~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25°C), 2% SDS, 1 mM reducing reagent, 3.6 M Urea, and stabilizer.

Quality Control: Tested in polyacrylamide gel electrophoresis and Western Blotting.