This modified BCA Low Protein Assay system greatly increases the sensitivity of protein quantitation. The principle, however, stays the same as the standard bicinchoninic acid assay. Briefly, Cu2+-protein complex formed under alkaline conditions reduces the Cu2+ to Cu1+. BCA binds the Cu+1, resulting in a purple colored complex, which is measured at 562 nm to determine the protein concentration.

Protein + Cu2+ => Cu1+ + BCA => Cu1+- BCA complex

Any protein containing cysteine, cysteine, tryptophan, tyrosine, and the peptide bond can reduce Cu2+ to Cu1+.

SPECIAL FEATURES

• Simple and highly sensitive for assay proteins at low concentration.
  
• Concentration range for protein assay from 1.0-100µg/ml.
  
• Compatible with many ionic and non-ionic detergents.
  
• Independent of specific amino acid content of proteins.
  
• Good for assays using cuvettes or a micro-plate format.
  

KIT CONTENTS

• BCA Low Protein Reagent A: 125ml
  
• BCA Low Protein Reagent B: 125ml
  
• BCA Low Protein Reagent C: 7.5ml
  
• Protein Standard Solution: 5ml