PreSafeStain 6X DNA Loading Dye
This modified BCA Low Protein Assay system greatly increases the sensitivity of protein quantitation. The principle, however, stays the same as the standard bicinchoninic acid assay. Briefly, Cu2+-protein complex formed under alkaline conditions reduces the Cu2+ to Cu1+. BCA binds the Cu+1, resulting in a purple colored complex, which is measured at 562 nm to determine the protein concentration.
Protein + Cu2+ => Cu1+ + BCA => Cu1+- BCA complex
Any protein containing cysteine, cysteine, tryptophan, tyrosine, and the peptide bond can reduce Cu2+ to Cu1+.
|Catalog #||Name||Size||Unit Price||Qty|
|WM1000||6. Western Protein Marker||250ul||$199.00|