
PreSafeStain 6X DNA Loading Dye
$98.00
View ProductThe principle of the bicinchoninic acid (BCA) assay is similar to the Lowry procedure, in that both rely on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. In the second step, BCA forms a purple colored complex with Cu+1, which is detectable at 562 nm.
Protein + Cu2+ => Cu1+ + BCA => Cu1+- BCA complex
It has been shown that cysteine, cystosine, tryptophan, tyrosine, and the peptide bond are able to reduce Cu2+ to Cu1+. BCA forms a purple-blue complex with Cu1+ in alkaline environments, thus providing a basis to monitor the reduction of alkaline Cu2+ by proteins at absorbance maximum 562 nm.
SPECIAL FEATURES
KIT CONTENTS
Size | Unit Price | Qty | ||
---|---|---|---|---|
1 kit |
$175.00 |
Catalog # | Name | Size | Unit Price | Qty | ||
---|---|---|---|---|---|---|
G1002 | BCA Protein Assay Kit | 1 kit | $175.00 | |||
PROT-SC | Shredder Spin Column With Cap | 100/set | $108.00 | |||
PROT-SC-N | Shredder Spin Column | 100/set | $108.00 | |||
G003 | 8. Protein Gel SafeStain- Comparable to SYPRO™ Ruby | for 1 liter | $269.00 | |||
WM1000 | 6. Western Protein Marker | 250ul | $199.00 | |||
G02103 | 3. AccuRuler Broad Prestained Protein Ladder | 2 x 250ul | $198.00 |