PreSafeStain 6X DNA Loading Dye
High performance, ready-to-use 2X PCR master mix with HotStart Taq DNA polymerase, in green color. Users only need to add template, primers and H2O for the reaction. The HotStart PCR 2x Master Mix contains inert, non-toxic dyes to visualize PCR mixing step, and also allows direct loading of PCR products on to gels for electrophoresis.
The HotStart Taq DNA Polymerase is a chemically modified Taq DNA Polymerase, whose enzyme activities can only be activated after 3-5 minutes of incubation at 95°C. The HotStart Taq Polymerase uses amplification conditions for regular Taq DNA Polymerase, except no polymerase activity will be present before the onset of thermal cycling. This prevents nonspecific DNA amplification and primer dimer formation. The amplified products, up to 7Kb in length, contain PCR products with blunt and 3’-overhanging A end. This allows flexible protocols for PCR cloning, if the amplified fragments need to be cloned.