PreSafeStain 6X DNA Loading Dye
Tsg DNA Polymerase is a thermostable DNA polymerase isolated from a strain of Thermus sp., and designed for primer extension reactions and PCR. Tsg has a half-life of 3 hours at 95oC, which means it is more stable than other DNA polymerases. It is also a high fidelity polymerase, with an error frequency of 15x106 during DNA synthesis. DNA sequencing with Tsg DNA Polymerase produces uniform band intensities and low background. Tsg has independent terminal transferase activity, which results in the addition of a single adenosine (A) at the 3' end of the extension product, thereby allowing the amplification product to be cloned in T-vectors.