Description:

The miRNA All-In-One cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA of miRNA from total RNA templates. This optimized kit comes with two components, the 2X miRNA cDNA Synthesis SuperMix and Enzyme Mix, enabling a hassle-free set-ups with minimal potential for contamination and more quantitatively accuracy due to significantly reduced pipetting or small volume reagents and samples. The kit contains all miRNA synthesis-required reagents including Poly(A) Polymerase and Reverse Transcriptase, a unique miRNA Oligo d(T) adapter that anneals to the A-tailed miRNA template, and RNaseOFF Ribonuclease Inhibitor to protect RNA template from degradation.

Advantages:

• Convenience: one tube, one reaction, one pipetting for RT reagents.
• Accuracy: ensures sample to sample consistency.
• Capacity: capable for any complex RNA templates.
• Flexibility: large RNA sample or reaction volume.
• Sensitivity: work as well at low concentration of RNA samples.